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gfp d2gfp reporter  (Addgene inc)


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    Addgene inc gfp d2gfp reporter
    Fig. 2 | Split-engineered BEs represent a generalizable strategy to enable small-molecule-controlled editing. a, Schematics of a traditional intact BE in the BE4max scaffold and the seBE strategy, including chemically induced dimerization of FRB and FKBP12 by rapamycin. b, Editing efficiency can be evaluated in a HEK293T cell line containing a single copy of integrated, constitutively expressed <t>d2gfp.</t> The presence of d2gfp-targeting sgRNA can introduce a stop codon (Q158*) and abrogate fluorescence to generate GFPoff cells, which can be tracked by either flow cytometry or deep sequencing of the locus. c, Representative flow cytometry histograms associated with transfection of intact or seBE constructs in the presence or absence of rapamycin. d, Mean and standard deviation for quantification of GFPoff cells by flow cytometry, with individual data points shown. A two-sided Mann–Whitney test was performed to compare intact and seBE GFPoff% (*P ≤ 0.05; **P ≤ 0.01). Exact P values provided as statistical source data files. NA, not applicable. e, Left shows deep-sequencing results demonstrating C to T conversion efficiency of the Q158 target cytosine under conditions identical to d. The mean and standard deviation are noted, with individual data points shown. Fold change (FC) is the ratio of mean values for the higher versus the lower condition in each comparison. Two-sided Mann–Whitney test was performed (NS, not significant; *P ≤ 0.05; **P ≤ 0.01). Right shows the editing footprints across the d2gfp locus for each condition. The full targeting sequencing is provided with the sgRNA protospacer (black) starting 20 bp from the protospacer adjacent motif (yellow) and the target C highlighted in red. In the editing footprint, the target cytosine base within the Q158 codon is noted with a blue arrow. Data represent position-wise averages of three or more biological replicates, with individual replicate data provided in Supplementary Table 1. Exact P values are provided as statistical source data files.
    Gfp D2gfp Reporter, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gfp d2gfp reporter/product/Addgene inc
    Average 93 stars, based on 25 article reviews
    gfp d2gfp reporter - by Bioz Stars, 2026-05
    93/100 stars

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    1) Product Images from "Controllable genome editing with split-engineered base editors."

    Article Title: Controllable genome editing with split-engineered base editors.

    Journal: Nature chemical biology

    doi: 10.1038/s41589-021-00880-w

    Fig. 2 | Split-engineered BEs represent a generalizable strategy to enable small-molecule-controlled editing. a, Schematics of a traditional intact BE in the BE4max scaffold and the seBE strategy, including chemically induced dimerization of FRB and FKBP12 by rapamycin. b, Editing efficiency can be evaluated in a HEK293T cell line containing a single copy of integrated, constitutively expressed d2gfp. The presence of d2gfp-targeting sgRNA can introduce a stop codon (Q158*) and abrogate fluorescence to generate GFPoff cells, which can be tracked by either flow cytometry or deep sequencing of the locus. c, Representative flow cytometry histograms associated with transfection of intact or seBE constructs in the presence or absence of rapamycin. d, Mean and standard deviation for quantification of GFPoff cells by flow cytometry, with individual data points shown. A two-sided Mann–Whitney test was performed to compare intact and seBE GFPoff% (*P ≤ 0.05; **P ≤ 0.01). Exact P values provided as statistical source data files. NA, not applicable. e, Left shows deep-sequencing results demonstrating C to T conversion efficiency of the Q158 target cytosine under conditions identical to d. The mean and standard deviation are noted, with individual data points shown. Fold change (FC) is the ratio of mean values for the higher versus the lower condition in each comparison. Two-sided Mann–Whitney test was performed (NS, not significant; *P ≤ 0.05; **P ≤ 0.01). Right shows the editing footprints across the d2gfp locus for each condition. The full targeting sequencing is provided with the sgRNA protospacer (black) starting 20 bp from the protospacer adjacent motif (yellow) and the target C highlighted in red. In the editing footprint, the target cytosine base within the Q158 codon is noted with a blue arrow. Data represent position-wise averages of three or more biological replicates, with individual replicate data provided in Supplementary Table 1. Exact P values are provided as statistical source data files.
    Figure Legend Snippet: Fig. 2 | Split-engineered BEs represent a generalizable strategy to enable small-molecule-controlled editing. a, Schematics of a traditional intact BE in the BE4max scaffold and the seBE strategy, including chemically induced dimerization of FRB and FKBP12 by rapamycin. b, Editing efficiency can be evaluated in a HEK293T cell line containing a single copy of integrated, constitutively expressed d2gfp. The presence of d2gfp-targeting sgRNA can introduce a stop codon (Q158*) and abrogate fluorescence to generate GFPoff cells, which can be tracked by either flow cytometry or deep sequencing of the locus. c, Representative flow cytometry histograms associated with transfection of intact or seBE constructs in the presence or absence of rapamycin. d, Mean and standard deviation for quantification of GFPoff cells by flow cytometry, with individual data points shown. A two-sided Mann–Whitney test was performed to compare intact and seBE GFPoff% (*P ≤ 0.05; **P ≤ 0.01). Exact P values provided as statistical source data files. NA, not applicable. e, Left shows deep-sequencing results demonstrating C to T conversion efficiency of the Q158 target cytosine under conditions identical to d. The mean and standard deviation are noted, with individual data points shown. Fold change (FC) is the ratio of mean values for the higher versus the lower condition in each comparison. Two-sided Mann–Whitney test was performed (NS, not significant; *P ≤ 0.05; **P ≤ 0.01). Right shows the editing footprints across the d2gfp locus for each condition. The full targeting sequencing is provided with the sgRNA protospacer (black) starting 20 bp from the protospacer adjacent motif (yellow) and the target C highlighted in red. In the editing footprint, the target cytosine base within the Q158 codon is noted with a blue arrow. Data represent position-wise averages of three or more biological replicates, with individual replicate data provided in Supplementary Table 1. Exact P values are provided as statistical source data files.

    Techniques Used: Introduce, Fluorescence, Flow Cytometry, Sequencing, Transfection, Construct, Standard Deviation, MANN-WHITNEY, Comparison



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    gfp d2gfp reporter  (Addgene inc)
    93
    Addgene inc gfp d2gfp reporter
    Fig. 2 | Split-engineered BEs represent a generalizable strategy to enable small-molecule-controlled editing. a, Schematics of a traditional intact BE in the BE4max scaffold and the seBE strategy, including chemically induced dimerization of FRB and FKBP12 by rapamycin. b, Editing efficiency can be evaluated in a HEK293T cell line containing a single copy of integrated, constitutively expressed <t>d2gfp.</t> The presence of d2gfp-targeting sgRNA can introduce a stop codon (Q158*) and abrogate fluorescence to generate GFPoff cells, which can be tracked by either flow cytometry or deep sequencing of the locus. c, Representative flow cytometry histograms associated with transfection of intact or seBE constructs in the presence or absence of rapamycin. d, Mean and standard deviation for quantification of GFPoff cells by flow cytometry, with individual data points shown. A two-sided Mann–Whitney test was performed to compare intact and seBE GFPoff% (*P ≤ 0.05; **P ≤ 0.01). Exact P values provided as statistical source data files. NA, not applicable. e, Left shows deep-sequencing results demonstrating C to T conversion efficiency of the Q158 target cytosine under conditions identical to d. The mean and standard deviation are noted, with individual data points shown. Fold change (FC) is the ratio of mean values for the higher versus the lower condition in each comparison. Two-sided Mann–Whitney test was performed (NS, not significant; *P ≤ 0.05; **P ≤ 0.01). Right shows the editing footprints across the d2gfp locus for each condition. The full targeting sequencing is provided with the sgRNA protospacer (black) starting 20 bp from the protospacer adjacent motif (yellow) and the target C highlighted in red. In the editing footprint, the target cytosine base within the Q158 codon is noted with a blue arrow. Data represent position-wise averages of three or more biological replicates, with individual replicate data provided in Supplementary Table 1. Exact P values are provided as statistical source data files.
    Gfp D2gfp Reporter, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gfp d2gfp reporter/product/Addgene inc
    Average 93 stars, based on 1 article reviews
    gfp d2gfp reporter - by Bioz Stars, 2026-05
    93/100 stars
    		  Buy from Supplier

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    Fig. 2 | Split-engineered BEs represent a generalizable strategy to enable small-molecule-controlled editing. a, Schematics of a traditional intact BE in the BE4max scaffold and the seBE strategy, including chemically induced dimerization of FRB and FKBP12 by rapamycin. b, Editing efficiency can be evaluated in a HEK293T cell line containing a single copy of integrated, constitutively expressed d2gfp. The presence of d2gfp-targeting sgRNA can introduce a stop codon (Q158*) and abrogate fluorescence to generate GFPoff cells, which can be tracked by either flow cytometry or deep sequencing of the locus. c, Representative flow cytometry histograms associated with transfection of intact or seBE constructs in the presence or absence of rapamycin. d, Mean and standard deviation for quantification of GFPoff cells by flow cytometry, with individual data points shown. A two-sided Mann–Whitney test was performed to compare intact and seBE GFPoff% (*P ≤ 0.05; **P ≤ 0.01). Exact P values provided as statistical source data files. NA, not applicable. e, Left shows deep-sequencing results demonstrating C to T conversion efficiency of the Q158 target cytosine under conditions identical to d. The mean and standard deviation are noted, with individual data points shown. Fold change (FC) is the ratio of mean values for the higher versus the lower condition in each comparison. Two-sided Mann–Whitney test was performed (NS, not significant; *P ≤ 0.05; **P ≤ 0.01). Right shows the editing footprints across the d2gfp locus for each condition. The full targeting sequencing is provided with the sgRNA protospacer (black) starting 20 bp from the protospacer adjacent motif (yellow) and the target C highlighted in red. In the editing footprint, the target cytosine base within the Q158 codon is noted with a blue arrow. Data represent position-wise averages of three or more biological replicates, with individual replicate data provided in Supplementary Table 1. Exact P values are provided as statistical source data files.

    Journal: Nature chemical biology

    Article Title: Controllable genome editing with split-engineered base editors.

    doi: 10.1038/s41589-021-00880-w

    Figure Lengend Snippet: Fig. 2 | Split-engineered BEs represent a generalizable strategy to enable small-molecule-controlled editing. a, Schematics of a traditional intact BE in the BE4max scaffold and the seBE strategy, including chemically induced dimerization of FRB and FKBP12 by rapamycin. b, Editing efficiency can be evaluated in a HEK293T cell line containing a single copy of integrated, constitutively expressed d2gfp. The presence of d2gfp-targeting sgRNA can introduce a stop codon (Q158*) and abrogate fluorescence to generate GFPoff cells, which can be tracked by either flow cytometry or deep sequencing of the locus. c, Representative flow cytometry histograms associated with transfection of intact or seBE constructs in the presence or absence of rapamycin. d, Mean and standard deviation for quantification of GFPoff cells by flow cytometry, with individual data points shown. A two-sided Mann–Whitney test was performed to compare intact and seBE GFPoff% (*P ≤ 0.05; **P ≤ 0.01). Exact P values provided as statistical source data files. NA, not applicable. e, Left shows deep-sequencing results demonstrating C to T conversion efficiency of the Q158 target cytosine under conditions identical to d. The mean and standard deviation are noted, with individual data points shown. Fold change (FC) is the ratio of mean values for the higher versus the lower condition in each comparison. Two-sided Mann–Whitney test was performed (NS, not significant; *P ≤ 0.05; **P ≤ 0.01). Right shows the editing footprints across the d2gfp locus for each condition. The full targeting sequencing is provided with the sgRNA protospacer (black) starting 20 bp from the protospacer adjacent motif (yellow) and the target C highlighted in red. In the editing footprint, the target cytosine base within the Q158 codon is noted with a blue arrow. Data represent position-wise averages of three or more biological replicates, with individual replicate data provided in Supplementary Table 1. Exact P values are provided as statistical source data files.

    Article Snippet: HEK293T cells were lentivirally transduced with a constitutively expressed destabilized GFP (d2GFP) reporter (derived from Addgene no. 14760) and selected for individual clones that contained a single copy of integrated d2gfp.

    Techniques: Introduce, Fluorescence, Flow Cytometry, Sequencing, Transfection, Construct, Standard Deviation, MANN-WHITNEY, Comparison